Biology News
Major Ireland tourism initiative to turn national parks into world class visitor experiences - Blooloop


Blooloop
“They are truly the Wild Heart of Ireland and each site contains unique habitats, flora and fauna, as well as dramatically different landscapes.” She said the aim was not only to attract more visitors but also to “ensure the long term protection of the ...

New faculty member studies the mechanics of development, challenging long-held assumptions - The Rockefeller University Newswire


The Rockefeller University Newswire
Amy Shyer has nothing against genes. The bulk of her scientific training, in fact, took place in molecular biology and genetics departments. However, as she investigates the early stages of organ formation, she hopes to divert a bit of attention away ...

The Anatomy Of AMD And Micron's Short Sellers - Seeking Alpha


Seeking Alpha
Without a long or short thesis, I provided some understanding of how AMD and Micron's short traders place their trades within a 24-hour period. AMD and Micron's shorts are best in trading in the after-hours session in order to get the best prices. AMD ...

Long PCR Protocol

Southborough - United States

Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA

Related
PCR Protocol
United States

Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.

Colony PCR protocol
United States

Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.

Inverse PCR Protocol
United States

Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.

Quantitative PCR Protocol
United States

From the Jackson Laboratory, University of Maine, USA.

Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)
United States

Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.

Gene Quantification and real-time PCR or kinetic RT-PCR
Germany

News- and overview site on technical aspects of gene expression analysis with real-time RT-PCR and competitive RT-PCR from the Technical University of Munich, Germany

PCR Amplification of cDNA Segments by 2 Stage Nested PCR
United States

Protocol from the method database of NIH, USA.

Effect of PCR Buffer on Multiplex PCR
Germany

Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company). [PDF]

PCR and Multiplex PCR Guide
United States

Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.

AFLP Protocol
United States

Detailed protocol by S Hazen, Wheat Breeding and Genetics, Michigan State University, USA.