Biology News
Taking ibuprofen for long periods found to alter human testicular physiology - Medical Xpress


Medical Xpress
A team of researchers from Denmark and France has found that taking regular doses of the pain reliever ibuprofen over a long period of time can lead to a disorder in men called compensated hypogonadism. In their paper published in Proceedings of the ...
IFLScience
USA TODAY
EconoTimes
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Jackson & Maggie Are Stuck in a Bathroom — Wearing Only Towels — in Grey's Anatomy Sneak Peek - PEOPLE.com


PEOPLE.com
he asks before he takes a seat next to her as they indefinitely wait. This moment adds fuel to the fire for fans who are wondering if Maggie is Jackosn's new love interest in the long-running ABC series. Despite their hopes, it might be difficult (and ...

Penn-led team uncovers the physiology behind the hour-long mating call of midshipman fish - EurekAlert (press release)


EurekAlert (press release)
A new study led by University of Pennsylvania researchers provides an explanation for this display of physiological fortitude. In an investigation featured on the cover of the Journal of General Physiology, the team discovered that the fish release ...

Long PCR Protocol

Southborough - United States

Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA

Related
PCR Protocol
United States

Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.

Colony PCR protocol
United States

Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.

Inverse PCR Protocol
United States

Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.

Quantitative PCR Protocol
United States

From the Jackson Laboratory, University of Maine, USA.

Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)
United States

Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.

Gene Quantification and real-time PCR or kinetic RT-PCR
Germany

News- and overview site on technical aspects of gene expression analysis with real-time RT-PCR and competitive RT-PCR from the Technical University of Munich, Germany

PCR Amplification of cDNA Segments by 2 Stage Nested PCR
United States

Protocol from the method database of NIH, USA.

Effect of PCR Buffer on Multiplex PCR
Germany

Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company). [PDF]

PCR and Multiplex PCR Guide
United States

Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.

AFLP Protocol
United States

Detailed protocol by S Hazen, Wheat Breeding and Genetics, Michigan State University, USA.