Biology News
NSF awards $6.4 million grant to Sevilleta Long-Term Ecological Research drylands site - National Science Foundation (press release)


National Science Foundation (press release)
To discover how environmental change is affecting dryland ecosystems, the National Science Foundation (NSF) has awarded a five-year, $6.4 million grant in new support of the Sevilleta Long-Term Ecological Research (LTER) site in central New Mexico.

New Leak Says 'Pokémon GO' Is Getting A Gen 4 'Evolution Event' Next Week - Forbes


Forbes
A long while a back a leaker perfectly predicted Pokémon GO's Water Festival before it happened. Now, the leaker is back, and his sources seem to be telling him something even more exciting is in the cards. That would be a new “Evolution Event,” which ...

Unisexual salamander evolution: A long, strange trip - Science Daily


Science Daily
The research appears in the journal Evolution. First, a bit about the unisexual Ambystoma salamander: They're female, and they reproduce mainly through cloning and the occasional theft of another salamander species' sperm, which the males of sexual ...

Long PCR Protocol

Southborough - United States

Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA

Related
PCR Protocol
United States

Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.

Colony PCR protocol
United States

Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.

Inverse PCR Protocol
United States

Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.

Quantitative PCR Protocol
United States

From the Jackson Laboratory, University of Maine, USA.

Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)
United States

Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.

Gene Quantification and real-time PCR or kinetic RT-PCR
Germany

News- and overview site on technical aspects of gene expression analysis with real-time RT-PCR and competitive RT-PCR from the Technical University of Munich, Germany

PCR Amplification of cDNA Segments by 2 Stage Nested PCR
United States

Protocol from the method database of NIH, USA.

Effect of PCR Buffer on Multiplex PCR
Germany

Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company). [PDF]

PCR and Multiplex PCR Guide
United States

Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.

AFLP Protocol
United States

Detailed protocol by S Hazen, Wheat Breeding and Genetics, Michigan State University, USA.